Blue bottle

Change a solution’s color with a shake of your hand!

20 minutes



  • Put on protective gloves and eyewear.
  • Conduct the experiment on the protective underlay.
General safety rules
  • Do not allow chemicals to come into contact with the eyes or mouth.
  • Keep young children, animals and those not wearing eye protection away from the experimental area.
  • Store this experimental set out of reach of children under 12 years of age.
  • Clean all equipment after use.
  • Make sure that all containers are fully closed and properly stored after use.
  • Ensure that all empty containers are disposed of properly.
  • Do not use any equipment which has not been supplied with the set or recommended in the instructions for use.
  • Do not replace foodstuffs in original container. Dispose of immediately.
General first aid information
  • In case of eye contact: Wash out eye with plenty of water, holding eye open if necessary. Seek immediate medical advice.
  • If swallowed: Wash out mouth with water, drink some fresh water. Do not induce vomiting. Seek immediate medical advice.
  • In case of inhalation: Remove person to fresh air.
  • In case of skin contact and burns: Wash affected area with plenty of water for at least 10 minutes.
  • In case of doubt, seek medical advice without delay. Take the chemical and its container with you.
  • In case of injury always seek medical advice.
Advice for supervising adults
  • The incorrect use of chemicals can cause injury and damage to health. Only carry out those experiments which are listed in the instructions.
  • This experimental set is for use only by children over 12 years.
  • Because children’s abilities vary so much, even within age groups, supervising adults should exercise discretion as to which experiments are suitable and safe for them. The instructions should enable supervisors to assess any experiment to establish its suitability for a particular child.
  • The supervising adult should discuss the warnings and safety information with the child or children before commencing the experiments. Particular attention should be paid to the safe handling of acids, alkalis and flammable liquids.
  • The area surrounding the experiment should be kept clear of any obstructions and away from the storage of food. It should be well lit and ventilated and close to a water supply. A solid table with a heat resistant top should be provided
  • Substances in non-reclosable packaging should be used up (completely) during the course of one experiment, i.e. after opening the package.

FAQ and troubleshooting

How warm should the water be?

The water can be anywhere from lukewarm to hot. Its temperature only influences the reaction rate. The warmer the water you use, the faster the solution will pale.

I added more than 5 drops of methylene blue. Should I start the experiment over?

No, don’t worry! Continue the experiment.

How long will the liquid be able to change colors?

The liquid will “work” as long as the bottle contains oxygen and lactose supply.

My solution isn’t turning blue anymore. Is there anything I can try?

The solution stops turning blue when there is no more oxygen in the bottle to oxidize the methylene blue. To refresh the bottle’s oxygen supply, remove the stopper for a few seconds, then put it snugly back in the bottle. As long as the solution still contains lactose, it should continue changing colors.

Step-by-step instructions

First, use Ca(OH)2 to create a basic solution, i.e. a solution with an excess of OH- particles. The reactions in this experiment will only take place in a basic medium.


Now add some methylene blue and lactose. Methylene blue likes to take electrons from other molecules. And the molecules of lactose, the sugar you find in milk, like to give their electrons away. A perfect match, right?


Mix everything thoroughly to help the methylene blue take electrons from the lactose.


Once methylene blue has its coveted electrons, it becomes colorless. But don't worry—you can easily make it blue again! You just need something to take the electrons from the colorless methylene blue. That something is actually already sealed in your bottle: it's the oxygen O2 in the air that’s taking up about half of the volume of the bottle. Just mix the O2 into the solution by shaking the bottle and watch!



Dispose of the reagents and solid waste together with household garbage. Pour solutions down the sink and wash with an excess of water.

Scientific description

Even though you can restore your bottle’s blue hue and leave it to pale again several times, sooner or later it will stop working. Buy why? If you take a look at the reactions occurring as a whole, you'll notice that it's basically electrons  going from lactose  to oxygen , with methylene blue  carrying them. But both lactose and oxygen change in the process, losing their ability to interact with the methylene blue. Eventually one of two things will inevitably happen: either lactose will run out, or oxygen will.

Although you can’t play with your bottle forever, you can “recharge” it easily. To refresh the oxygen supply, just open the bottle and let some fresh air in. As for the lactose, there’s an excess of it in the solution, so you likely won't run out of it anytime soon. Replenishing it, however, is as easy as adding some more. But what if you do run out of lactose? Other sugars can come to the rescue! Some sugars, like ordinary table sugar, won’t work, but others, like glucose, will work just fine. Try using a little bit of honey or syrup instead of lactose and see what happens.

That’s interesting!

Why did we add an alkali to the lactose aqueous solution?

By adding calcium hydroxide Ca(OH)2 aqueous solution, we created an alkaline environment. Methylene blue needs an alkaline environment in order to accept electrons from lactose; otherwise, the reaction will not proceed, and the solution will remain blue. You can check this condition by conducting the experiment without Ca(OH)2.

Why is it so important to seal the bottle tightly?

First and foremost, you’ll be able to shake the bottle without sending any liquid flying.

Moreover, in sealing the bottle we are preventing ambient air from entering, and ensuring that the oxygen in the ambient air will not have access to our solution either. This is why the color can only be restored by shaking the bottle (see Why does the solution turn blue again?). The most diligent observers may notice that the blue tint doesn’t disappear completely after the first shake, but remains at the border between the solution and air in the bottle (along the so-called meniscus) and forms a nice blue fringe. The same would happen if the bottle were left open. This is caused by a high concentration of oxygen present in the air above the solution. The oxygen permeates the liquid-gas interface and converts methylene blue to its colored form. However, as the oxygen supply in the bottle is gradually depleted, this border gets increasingly thinner and finally disappears.